Production of High Activity Amylase from Trichoderma Reesei Ncim 1052 In Solid State Fermentation
نویسندگان
چکیده
The fungus, Trichoderma reesei NCIM 1052 was used for the production of extra cellular amylase on agricultural residues viz. Soy cake, Wheat bran, Rice bran and Ragi (Eleusine corocana Gaertn.) in solid state fermentation (SSF). Maximum enzyme activity (324.45 IU g-1) was obtained in a medium having Soy cake as the substrate, moisture level 1:1 (distilled water) and incubation for 72 hours with Tween-80 (0.05%) as the extractant. During the fermentation, starch was hydrolyzed completely with a corresponding increase in the biomass and amylase activity within 3 days. The optimum pH and Temperature of the crude enzyme was 5.0 and 50 0 C respectively.
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